JRC GMO-Amplicons | EURL GMFF

Record ID:	ON409199.1
Database:	nt	Go to original record
Description:	Lilium souliei chloroplast, complete genome.
Taxon:	Lilium souliei
Original size:	153164 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45539	46144	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttataa tataaaacta caataaaaaa agataggtgc agagactcaa
201   tggaagctgt tctaacgaat gaagttgatt acgttgcgct ggtagccgga
251   atccttctat caaaattacg gagaggatgc ccgccctata tacgtatata
301   tacatactga catagtataa cgattgatta atcgcggccc gaatccatta
351   taatatatat agatatgaaa aatttaagag ttattgtaaa tctataattc
401   caatcaaagt ttaaggaaga atcaaatatt cagtgatcaa attattcatt
451   ccagagtctg tatagatctt tttaaaaact gattattcgg acgagaataa
501   agagagagtc ccattctacg tgtcaatacc gacaacaatg aaatttatag
551   taaaaggaaa atccgtcgac tttataagtc gtgagggttc aagtccctct
601   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details