JRC GMO-Amplicons | EURL GMFF

Record ID:	OM403712.1
Database:	nt	Go to original record
Description:	Phytolacca dioica chloroplast, complete genome.
Taxon:	Phytolacca dioica
Original size:	155364 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47599	48217	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtacggaa
51    acctactaag tgaaaatttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa ctcctttttt tcaaaagaaa aaaataaggg
151   ttcagaaagc aagaaaaaaa ggataggtgc agagactcaa aggaagctgt
201   tctaacaaat ggggttgact gtgctgtgtt gttataagaa ttcttccata
251   aaaattccaa tccaaaaaaa aagggtaaag catatacgta ctgaactata
301   tcaaataatt aataacaacc cgaatcctta ctttactttt tttctaattt
351   ttatatattt atgaaattca aaaatttata tgaaaaaaaa aaatggaaga
401   atttttgtga atcgattcca aattgaaaga agaatcaaat attcattcat
451   caaataattc actccatagt ctgatagatt ttttgaagaa ctaattaatc
501   ggacgagaat gaagatagag tcccgttcta catgtcaata ctgacaacaa
551   tgaaatttat aattaagaag aaaatccgtc gactttaaaa atcgtgaggg
601   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details