JRC GMO-Amplicons | EURL GMFF

Record ID:	OK247960.1
Database:	nt	Go to original record
Description:	Phleum pratense subsp. nodosum trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Phleum pratense subsp. nodosum
Original size:	1778 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	829	1438	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaagggggtt ctcgaactag
151   aatacaaagg aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatcgagtt aattacgttg tgttgttagt gttagtggga ttccttctaa
251   attcgagaaa gaagggcttt atacatctaa taaacacgta tagatactga
301   tagcaaacga ttaatcacag aacccatatt ataatatagg ttctttattc
351   ttttttagaa tgaaatagaa atgattatga aataaaaaat tcagaatttt
401   tttagaatta ttgtgaatcc attccaatcg aatatttagt aatcaaatcc
451   ttcaattcaa agttttgagg tctttaaaaa agtggattaa tcggacgagg
501   acaaagagag agtcccattc tacatgtcaa tactgacaac aatgaaattt
551   ctagtaaaag gaaaatccgt cgactttata agtcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details