JRC GMO-Amplicons | EURL GMFF

Record ID:	OK247959.1
Database:	nt	Go to original record
Description:	Phleum montanum trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Phleum montanum
Original size:	1770 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	829	1434	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaagggggtt ctcgaactag
151   aatacaaagg aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatcgagtt aattacgttg tgttgttagt ggaattcctt ctaaattcga
251   gaaagaaggg ctttatacat ctaataaaca cgtatagata ctgacatagc
301   aaacgattaa tcacagaacc catattataa tataggttct ttattctttt
351   ttagaatgaa atagaaatga ttatgaaata aaaaattcag aattttttta
401   gaattattgt gaatccattc caatcgaata tttagtaatc aaatccttca
451   attcaaagtt ttgaggtctt taaaaaagtg gattaatcgg acgaggacaa
501   agagagagtc ccattctaca tgtcaatact gacaacaatg aaatttctag
551   taaaaggaaa atccgtcgac tttataagtc gtgagggttc aagtccctct
601   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details