JRC GMO-Amplicons | EURL GMFF

Record ID:	OK247950.1
Database:	nt	Go to original record
Description:	Catabrosella araratica trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Catabrosella araratica
Original size:	1703 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	813	1419	+	607 bp

Amplicon:

607 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaaccagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttctaaa
251   ttcgagaagg aagggcttta tacatctaat acctatagat actgccatag
301   caaacgatta atcacagaac ccatattata atataggttc tttattcttt
351   tttaaaatga aatagaaatg attatgaaat aaaaaattca taattttttt
401   agaattattg ggaatccatt cccatcgaat atttagtaat caaatccttc
451   aattcaaagt tttggggtct ttaaaaaagt agattaatcg aacgaggaca
501   aagagagagt cccattctac atgtcaatac tgacaacaat gaaatttcta
551   gtaaaaggaa aatccgtcga ctttataagt cgtgagggtt caagtccctc
601   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	607	588	2.73e-08

Match details