JRC GMO-Amplicons | EURL GMFF

Record ID:	OK247943.1
Database:	nt	Go to original record
Description:	Catabrosella variegata subsp. variegata trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Catabrosella variegata subsp. variegata
Original size:	1640 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	756	1357	+	602 bp

Amplicon:

602 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaagggggtt ctagaactag
151   aatacaaagg aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatcgagtt aatttgtgtt gttagtggaa ttccttctaa attcgagaag
251   gaagggcttt atacatctaa taaataccta tagatactgc catagcaaac
301   gattaatcac agaacccata ttataatata ggttttttat tcttttttag
351   aatgaaatag aaatgattat gaaataaaaa attcataatt tttttagaat
401   tattgtgaat ccattcccat cgaatattta gtaatcaaat ccttcaattc
451   aaagttttga ggtctttaaa aaaggagatt aatcgaacga ggacaaagag
501   agagtcccat tctacatgtc aatactgaca acaatgaaat ttctagtaaa
551   aggaaaatcc gtcgacttta taagtcgtga gggttcaagt ccctctatcc
601   cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	602	583	2.71e-08

Match details