JRC GMO-Amplicons | EURL GMFF

Record ID:	OK247940.1
Database:	nt	Go to original record
Description:	Bellardiochloa polychroa trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Bellardiochloa polychroa
Original size:	1798 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	843	1462	+	620 bp

Amplicon:

620 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttctaaa
251   ttcgagaaag aagggcttta tacatctaat aaacacgtat agatactgac
301   atagcaaacg attaatcaca gaacctatat tataatatat ggttctttat
351   tcttttttag aatgaaattt gaaatagaaa tgattatgaa ataaaaaatt
401   cataattttt tttagaatta ttgtgaatac attccaatcg aatatttagt
451   aatcaaatgc ttccattcaa agtttttagg tctttaaaaa agtggattaa
501   tcggacgagg acaaagagag agtcccattc tacatgtcaa tactgacaac
551   aatgaaattt ctagtaaaag gaaaatccgt cgactttata agtcgtgagg
601   gttcaagtcc ctctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	620	601	2.79e-08

Match details