JRC GMO-Amplicons | EURL GMFF

Record ID:	OK247931.1
Database:	nt	Go to original record
Description:	Alopecurus tiflisiensis trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Alopecurus tiflisiensis
Original size:	1752 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	796	1416	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaagggggtt ctcgaactag
151   aatacaaagg aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatcgagtt aattatgttg tgttgttagt ggaattcctt ctaaattcga
251   gaaagaaggg ctttatacat ctaataaaca cgtatagata ctgacatagc
301   aaacgattaa tcacagaacc tatattataa ttataatata tggttcttta
351   ttctttttta gaatgaaata gaaattatta tgaaataaaa aattcataat
401   ttttttttta gaattattgt gaatacattc caatcgaata tttagtaatc
451   aaatgcttca attcaaagtt ttgaggtctt taatttaaaa aagtggatta
501   atcggacgag gacaaagaga gagtcccatt ctacatgtca atactgacaa
551   caatgaaatt tctagtaaaa ggaaaatccg tcgactttat aagtcgtgag
601   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details