JRC GMO-Amplicons | EURL GMFF

Record ID:	OK247927.1
Database:	nt	Go to original record
Description:	Alopecurus magellanicus subsp. glaucus trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Alopecurus magellanicus subsp. glaucus
Original size:	1766 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	816	1430	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtattg ttagtggaat tccttctaaa
251   ttcgagaaag aagggcttta tacatgtaat aaacacgtat agatactgac
301   atagcaaacg attaatcaca gaacctatat tataatatat ggttctttat
351   tcttttttag aatgaaatag aaatgattat gaaataaaaa attcataatt
401   ttttttttag aattattgtg aatacattcc aatctaatat ttagtaatca
451   aatgcttcaa ttcaaagttt tgaggtcttt aaaaaagtgg attaatcgga
501   cgaggacaaa gagagagtcc cattctacat gtcaatactg acaacaatga
551   aatttctagt aaaaggaaaa tccgtcgact ttataagtcg tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details