JRC GMO-Amplicons | EURL GMFF

Record ID:	OK247925.1
Database:	nt	Go to original record
Description:	Alopecurus creticus trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Alopecurus creticus
Original size:	1782 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	826	1439	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtattttga gaaaacctgg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtattg ttagtggaat tccttctaaa
251   ttcgagaaag aagggcttta tacatgtaat aaacacgtat agatactgac
301   atagcaaacg attaatccca gaacccatat tataatatat ggttctttat
351   tcttttttag aatgaaatag aaatgattat gaaaaaaaaa ttatgaattt
401   tttttttaga attattgtga atacattcca atggaatctt tagtaatcaa
451   atgcttcaat tcaaagtttt gaggtcttta aaaaagtgga ttaatcggac
501   gaggacaaag agagagtccc attctacatg tcaatactga caacaatgaa
551   atttctagta aaaggaaaat ccgtcgactt tataagtcgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details