JRC GMO-Amplicons | EURL GMFF

Record ID:	HG970789.1
Database:	nt	Go to original record
Description:	Ixora sp. Alejandro 12-406 chloroplast trnT-trnL intergenic spacer, partial sequence, tRNA-Leu gene, complete sequence, and trnL-trnF intergenic spacer, partial sequence, specimen voucher USTH:5915
Taxon:	Ixora sp. Alejandro 12-406
Original size:	1627 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	714	1273	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattctgc gaaacccggg atctaattaa
101   aaaatcctga gccaaatcct gttttccgaa accaaaggta cagaaagtga
151   aaaggggata ggtgcagaga ctcaacggaa gttgttctaa caaatggagt
201   tggctgcgtt agtagagaaa tctttccatc gaaaattccg aaaggataaa
251   gtgaaagata aaggtatata cgtattgaat actatatcaa atgattaatg
301   acgactcgac tgaatctgta ttttttatat gaaaacggaa gaattggtgt
351   gaatagattc cacattaaag aaagaatcga atattcattg attaaatgat
401   tcactccata gtctgataga tcttttcaag aattgattaa tcggacgaga
451   ataaagatag agccccgttc tacatgtcaa tgtcggcaac aatgaaattt
501   atagtaagag gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details