JRC GMO-Amplicons | EURL GMFF

Record ID:	HG970781.1
Database:	nt	Go to original record
Description:	Ixora philippinensis chloroplast trnT-trnL intergenic spacer, partial sequence, tRNA-Leu gene, complete sequence, and trnL-trnF intergenic spacer, partial sequence, specimen voucher PNH:DT548
Taxon:	Ixora philippinensis
Original size:	1577 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	711	1255	+	545 bp

Amplicon:

545 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaacccaat cctgagccaa
101   atcctgtttt ccgaaaccaa aggtacagaa agtgaaaagg ggataggtgc
151   agagactcaa cggaagttgt tctaacaaat ggagttggct gcgttagtag
201   agaaatcttt ccatcgaaaa ttccgaaagg ataaagtgaa agataaaggt
251   atatacgtat tgaatactat atcaaatgat taatgacgac tcgactgaat
301   ctgtattttt tatatgaaaa cggaagaatt ggtgtgaata gattccacat
351   taaagaaaga atcgaatatt cattgattaa atgattcact ccatagtctg
401   atagatcttt tcaagaattg attaatcgga cgagaataaa gatagagccc
451   cgttctacat gtcaatgtcg gcaacaatga aatttatagt aagaggaaaa
501   tccgtcgact ttaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.45e-08
primerB	100.0	20 bp	0	0	545	526	2.45e-08

Match details