JRC GMO-Amplicons | EURL GMFF

Record ID:	HG970772.1
Database:	nt	Go to original record
Description:	Ixora macrophylla chloroplast trnT-trnL intergenic spacer, partial sequence, tRNA-Leu gene, complete sequence, and trnL-trnF intergenic spacer, partial sequence, specimen voucher USTH:5840
Taxon:	Ixora macrophylla
Original size:	1626 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	730	1296	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat ccctgagcca aaatcctgtt ttccgaaacc aaaggtacag
151   aaagtgaaaa ggggataggt gcagagactc aacggaagtt gttctaacaa
201   atggagttgg ctgcgttagt agagaaatct ttccatcgaa aattccgaaa
251   ggataaagtg aaagataaag gtatatacgt attgaatact atatcaaatg
301   attaatgacg actcgactga atctgtattt tttatatgaa aacggaagaa
351   ttggtgtgaa tagattccac attaaagaaa gaatcgaata ttcattgatt
401   aaatgattca ctccatagtc tgatagatct tttcaagaat tgattaatcg
451   gacgagaata aagatagagc cccgttctac atgtcaatgt cggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details