JRC GMO-Amplicons | EURL GMFF

Record ID:	HG970770.1
Database:	nt	Go to original record
Description:	Ixora luzoniensis chloroplast trnT-trnL intergenic spacer, partial sequence, tRNA-Leu gene, complete sequence, and trnL-trnF intergenic spacer, partial sequence, specimen voucher USTH:5939
Taxon:	Ixora luzoniensis
Original size:	1370 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	604	1154	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtacggaa
51    accccctaag tgataacttt caaattcaga gaagccctgg aaaatcctga
101   gccaaatcct gttttccgaa accaaaggta cagaaaagtg aaaaggggat
151   aggtgcagag actcaacgga agttgttcta acaaatggag ttggctgcgt
201   tagtagagaa atctttccat cgaaaattcc gaaaggataa agtgaaagat
251   aaaggtatat acgtattgaa tactatatca aatgattaat gacgactcgg
301   ctgaatctgt attttttata tgaaaacgga agaattggtg tgaatagatt
351   ccacattaaa gaaagaatcg aatattcatt gattaaatga ttcactccat
401   agtctgatag atcttttcaa gaattgatta atcggacgag aataaagata
451   gagccccgtt ctacatgtca atgtcggcaa caatgaaatt tatagtaaga
501   ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details