JRC GMO-Amplicons | EURL GMFF

Record ID:	HG970760.1
Database:	nt	Go to original record
Description:	Ixora bartlingii chloroplast trnT-trnL intergenic spacer, partial sequence, tRNA-Leu gene, complete sequence, and trnL-trnF intergenic spacer, partial sequence, specimen voucher USTH:12-1008
Taxon:	Ixora bartlingii
Original size:	1517 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	738	1268	+	531 bp

Amplicon:

531 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcggt acagaaagtg aaaaggggat aggtgcagag actcaacgga
151   agttgttcta acaaatggag ttggctgcgt tagtagagaa atctttccat
201   cgaaaattcc gaaaggataa agtgaaagat aaaggtatat acgtattgaa
251   tactatatca aatgattaat gacgactcga ctgaatctgt attttttata
301   tgaaaacgga agaattggtg tgaatagatt ccacattaaa gaaagaatcg
351   aatattcatt gattaaatga ttcactccat agtctgatag atcttttcaa
401   gaattgatta atcggacgag aataaagata gagccccgtt ctacatgtca
451   atgtcggcaa caatgaaatt tatagtaaga ggaaaatccg tcgactttaa
501   aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	531	512	2.39e-08

Match details