JRC GMO-Amplicons | EURL GMFF

Record ID:	HG970757.1
Database:	nt	Go to original record
Description:	Ixora sp. CB-2014a chloroplast trnT-trnL intergenic spacer, partial sequence, tRNA-Leu gene, complete sequence, and trnL-trnF intergenic spacer, partial sequence, specimen voucher USTH:5914
Taxon:	Ixora sp. CB-2014a
Original size:	1651 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	740	1298	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaaaaaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggtacagaa
151   agtgaaaagg ggataggtgc agagactcaa cggaagttgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaagtgaa agataaaggt atatacgtat tgaatactat atcaaatgat
301   taatgacgac tcgactgaat ctgtattttt tatatgaaaa cggaagaatt
351   ggtgtgaata gattccacat taaagaaaga atcgaatatt cattgattaa
401   atgattcact ccatagtctg cttttcaaga attgattaat cggacgagaa
451   taaagataga gccccgttct acatgtcaat gtcggcaaca atgaaattta
501   tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details