JRC GMO-Amplicons | EURL GMFF

Record ID:	OL977687.1
Database:	nt	Go to original record
Description:	Mimulicalyx rosulatus isolate Miyi chloroplast, complete genome.
Taxon:	Mimulicalyx rosulatus
Original size:	153587 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47704	48272	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaagaa aggttcagaa
151   aacgaaaaaa gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgttggta gaggaatttt tccatcgaaa cttcagaaag
251   gatgaaggat aaacgtatct attgaatact atatcaaatg attaatgatg
301   gcccgaatct gtatctgtat tttttatttt aatatgaaaa aaaatgcaag
351   aattggtgtg aattgattcc acattgaaga aggaatcgaa tattccttca
401   tcaaatcatt cactccatag tccgagaaat cttttaaaga acggattaat
451   cggacgagaa taaagataga gtcccattct acatgtcaat accggcaaca
501   atgaaattta tagtaagagg aaaatccgtc gacttttaaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details