JRC GMO-Amplicons | EURL GMFF

Record ID:	OL875079.1
Database:	nt	Go to original record
Description:	Hoya sp. 8 WO-2022 chloroplast, complete genome.
Taxon:	Hoya sp. 8 WO-2022
Original size:	177198 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	53697	54246	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc aggttgagaa aacgaaaaca
151   aggataggtg cagagactcg acggaagctg ttctaacaaa tggagttggc
201   cgcgttggta gagaaatcct tccatcgaaa attcagaaag gatgaaagat
251   atacatacgt attgaatact atatcaaata attaatgccg acccaaatga
301   gtctgtattt tttctataaa aacggaagaa ttggtgtaat tcgattcttt
351   cttcaatgtg gaatcgaata ttcattgatc aaaagattca ctccatagtc
401   tgtagatcct cttttcaaga actggattaa tcggacgaga ataaagatag
451   agtcccgttc tacatgtcaa tgctggcaac aatgcaattt tgagtaagag
501   gaaaatccgt cgacttaaaa aatcgtgagg gttcaagtcc ctctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details