JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ374065.1
Database:	nt	Go to original record
Description:	Expression vector pBHg-FfNoxA-OE sequence.
Taxon:	Expression vector pBHg-FfNoxA-OE
Original size:	11584 bp

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Method:	QL-ELE-00-023	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of T35S pCAMBIA sequences (Rischitor et al., 2016).
Target:	T35S pCAMBIA

View other 1686 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
1	1	10507	10643	-	137 bp

Amplicon:

137 bp

1     acgggggtct ggattttagt actggatttt ggttttagga attagaaatt
51    ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta
101   tatgctcaac acatgagcga aaccctatag gaaccct

View other 7 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	95.2	21 bp	0	1	2	21	3.45e-07
primerB	100.0	23 bp	0	0	137	115	1.68e-10

Match details