JRC GMO-Amplicons | EURL GMFF

Record ID:	ON055287.1
Database:	nt	Go to original record
Description:	Aster fanjingshanicus chloroplast, complete genome.
Taxon:	Aster fanjingshanicus
Original size:	153225 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47517	48047	+	531 bp

Amplicon:

531 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcacgtttt ccgaaaacaa gcaaaggttc
151   agaaagcgaa aataaaaaag gataggtgca gagactcgat ggaagctatt
201   ctaacgaatg gagttaattg tatacattgg tataggaatc cttctatcga
251   aacttcagaa aagtgaagga taagcctgta tacataatac agaagaattg
301   gtgtgaatcg attccatatt gaagaaagaa tccaatatta atccaatatt
351   aatattaatt gatcaaatca ttcactccat aatctgatag atcttttgaa
401   gaactgatta atcggacgag aataaagata gagtcccgtt ctacatgtca
451   atattggcaa caatgaaatt tatagtaaga ggaaaatccg tcgatttcaa
501   aaatcatgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	531	512	2.39e-08

Match details