JRC GMO-Amplicons | EURL GMFF

Record ID:	MW752201.1
Database:	nt	Go to original record
Description:	Salvia sonchifolia voucher GX Hu et al. 0431 chloroplast, complete genome.
Taxon:	Salvia sonchifolia
Original size:	151470 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46644	47166	+	523 bp

Amplicon:

523 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaaaaa aaggataggt
151   gcagagactc aatggaagtt gttctaacaa atggagttga ctgcgccggt
201   agaggaatct ttccacggaa actttagaaa ggataaacgc atctattgaa
251   tactatatca aatgattaat gttggcccga atctgttttt ttaatatgaa
301   aatggaaaag tcgatgtgaa tttatttcac gttgaagaat aaatcgaata
351   ttcatcaact cattcactcc atagttcgat agatctttta aagaacttat
401   taatcggacg agaataaaga tagagtccca ttctacatgt caataccggc
451   aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg
501   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.35e-08
primerB	100.0	20 bp	0	0	523	504	2.35e-08

Match details