JRC GMO-Amplicons | EURL GMFF

Record ID:	OX344734.1
Database:	nt	Go to original record
Description:	Ballota nigra genome assembly, organelle: plastid:chloroplast
Taxon:	Ballota nigra
Original size:	151913 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46653	47215	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaatta taatgaaatt gagccttagt
51    gcgggaaacc aactaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttttc aaagcaaagg
151   ttcaaaaaac gaaataaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactgc gttggtagag gaatcctttc tacggaaact
251   tcagaaagga tgaaggataa ccgtatctat cgaatactat atcaaattat
301   taatgatggc ccgaatccgt attttttaat atgaaaaata gaagaattgg
351   tgtgaattga ttctatattg aagaaaaaat agaatattca ttcatcaaat
401   cattcactcc atagtccgat agatctttta aagaactgat taatcggacg
451   agaataaaga tagagtcccg ttctacatgt caatactggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details