JRC GMO-Amplicons | EURL GMFF

Record ID:	ON858917.1
Database:	nt	Go to original record
Description:	Isodon yuennanensis chloroplast, complete genome.
Taxon:	Isodon yuennanensis
Original size:	152265 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46837	47369	+	533 bp

Amplicon:

533 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaaaaa ggataggtgc
151   agagactcaa tggaagctgt tctaataaat gggagttgac tgcgctggta
201   gaggaatctt tccatggaaa ctttagaaag gatgaaggat aaacgcatct
251   attgaatact atatcaaatg gttaatgatg gcccgaatct tttaatatga
301   aaaaatggaa aaattggtgt gaattgattc tacgttgaag aaaaaatcaa
351   atattcatca actcattcgc tccatagtcc gatagatctt ttaaagaact
401   aattaatcgg acgagaataa agatagagtc ccattctaca tgctacatgt
451   caatatcggc aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt
501   aaaaatcgtg agggttcaag tccctctatc ccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.40e-08
primerB	100.0	20 bp	0	0	533	514	2.40e-08

Match details