JRC GMO-Amplicons | EURL GMFF

Record ID:	ON858903.1
Database:	nt	Go to original record
Description:	Isodon japonicus var. japonicus chloroplast, complete genome.
Taxon:	Isodon japonicus var. japonicus
Original size:	152684 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47084	47636	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaaaaa ggttcaaaaa
151   acgaaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tgggagttga ctgcgctggt agaggaatct ttccatggaa actttagaaa
251   ggatgaagga taaacgcatc atactatatc aaaatcaaat ggttaatgat
301   ggcccgaatc ttttaatata aaaaaatgga aaaattggtg tgaattgatt
351   ctacgttgaa gaaaaatcga atattcatca actcattcgc tccatagtcc
401   gatagatctt ttaaagaact gattaatcgg acgagaataa agatagagtc
451   ccattctaca tgctacatgt caatatcggc aacaatgaaa tttatagtaa
501   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
551   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details