JRC GMO-Amplicons | EURL GMFF

Record ID:	ON858886.1
Database:	nt	Go to original record
Description:	Isodon rubescens chloroplast, complete genome.
Taxon:	Isodon rubescens
Original size:	152651 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47114	47668	+	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaaaaa ggttcaaaaa
151   acgaaaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atgggagttg actgcgctgg tagaggaatc tttccatgga aactttagaa
251   aggatgaagg ataaacgcat catactatat caaaatcaaa tggttaatga
301   tggcccgaat cttttaatat aaaaaaatgg aaaaattggt gtgaattgat
351   tctacgttga agaaaaaatc gaatattcat caactcattc gctccatagt
401   ccgatagatc ttttaaagaa ctgattaatc ggacgagaat aaagatagag
451   tcccattcta catgctacat gtcaatatcg gcaacaatga aatttatagt
501   aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca agtccctcta
551   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details