JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ484962.1
Database:	nt	Go to original record
Description:	Tocoyena arenicola voucher Melo 7183 (ALCB) trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Tocoyena arenicola
Original size:	1670 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	712	1284	+	573 bp

Amplicon:

573 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagccg tgcttcggaa
51    accccactaa gtgaagtgat aactttcaaa ttcagagaaa ccctggaatt
101   aataaaaagg ggcaatcctg agccaaatcc cgttttccga aaccaaaggt
151   tcagaaagtg aaaaaaggat aggtgcagag actcaacgga agctgtttta
201   acaaatggag ttggctacgt tagtagagaa atctttccat ctaaaattcc
251   gaaaggataa agtgaaggat aaacgtatat atacgtattg aatactatat
301   caaatgatta atgacgactc gactgaatct gtatttttta tatgaaaacg
351   gaagaattgg tgtgaataga ttccacattg aagaaggaat cgaatattca
401   ttgatcaaat gattcactcc atagtctgat agatcttttc aagaattgat
451   taatcggacg agaataaaga tagagtcccg ttctacatgt caatgacggc
501   aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg
551   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	573	554	2.58e-08

Match details