JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ466561.1
Database:	nt	Go to original record
Description:	Tocoyena formosa voucher Borges 340 trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Tocoyena formosa
Original size:	1721 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	783	1354	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgaagtgata actttcaaat tcagagaaac cctggaatta
101   ataaaaaggg gcaatcctga gccaaatccc gttttccgaa accaaaggtt
151   cagaaagtga aaaaaggata ggtgcagaga ctcaacggaa gctgttctaa
201   caaatggagt tggctacgtt agtagagaaa tctttccatc taaaattccg
251   aaaggataaa gtgaaggata aacgtatata tacgtattga atactatatc
301   aaatgattaa tgacgactcg actgaatctg tattttttat atgaaaacgg
351   aagaattggt gtgaatagat tccacattga agaaggaatc gaatattcat
401   tgatcaaatg attcactcca tagtctgata gatcttttca agaattgatt
451   aatcggacga gaataaagat agagtcccgt tctacatgtc aatgtcggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details