JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ466552.1
Database:	nt	Go to original record
Description:	Tocoyena hispidula voucher Harley 56467 (ALCB) trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Tocoyena hispidula
Original size:	1675 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	726	1290	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt acgaaaccaa ggtcaaaaag
151   taaaaaaagg ataggtgcag agactcaacg gaagctgttc taacaaatgg
201   agttggctac gttagtagag aaatctttcc atctaaaatt ccgaaaggat
251   aaagtgaagg ataaacgtat atatacgtat tgaatactat atcaaatgat
301   taatgacgac tcggctgaat ctgtattttt tatatgaaaa cggaagaatt
351   ggtgtgaata gattccacat tgaagaagga atcgaatatt cattgatcaa
401   atgattcact ccatagtctg atagatcttt tcaagaattg attaatcgga
451   cgagaataaa gatagagtcc cgttctacat gtcaatgtcg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttacaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details