JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ458142.1
Database:	nt	Go to original record
Description:	Randia sp. Gomes 1393 voucher Gomes 1393 (ALCB) trnT-trnL intergenic spacer and tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Randia sp. Gomes 1393
Original size:	1657 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	704	1271	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt tcaaattcag agaaaccctg gaattaataa
101   aaaggggcaa tcctgagcca aatcccgttt ttccgaaacc aaaggttcag
151   aaagtgaaaa aggataggtg cagagactca acggaagctg ttctaacaaa
201   tggagttggc tgcgttagta gagaaatctt tccatctaaa attccgaaag
251   gataaagtga aggataaacg tatatatacg tattgaatac tatatcaaat
301   gattaatgac gactcgactg aatctgtatt ttttatatga aaacggaaga
351   attggtgtga atagattcca cattgaagaa ggaatcgaat attcattgat
401   caaatgattc actccatagt ttgatagatc ttttcaagaa ttgattaatc
451   ggacgagaat aaagatagag tcccgttcta catgtcaatg tcggcaacaa
501   tgaaatttat agtaagagga aaatccgtcg actttaaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details