JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ458132.1
Database:	nt	Go to original record
Description:	Randia armata voucher Rova 2393 (GB) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Randia armata
Original size:	1640 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	688	1254	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg aaatgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggctg cgttagtaga gaaatctttc catctaaaat ccgaaaggat
251   aaagtgaagg ataaacgtat atatacgtat tcaatactat atcaaatgat
301   taatgacgac tcgactgaat ctctgtattt tttatatgaa aacggaagaa
351   ttggtgtgaa taaattccac attgaagaag gaatcgaata ttcattgatc
401   aaatgattca ctccatagtc tgatagatct tttcaagaat tgattaatcg
451   gacgagaata aagatagagt cccgttctac atgtcaatgt cggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details