JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ458131.1
Database:	nt	Go to original record
Description:	Randia aculeata voucher Tuberquia 507 (GB) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Randia aculeata
Original size:	1686 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	731	1300	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt catcaaattc agagaaaccc tggaattaat
101   aaaaaggggc aatcctgagc caaatcccgt tttccgaaac caaaggttca
151   gaaagtgaaa aaaggatagg tgcagagact caacggaagc tgttctaaca
201   aatggagttg gctgcgttag tagagaaatc tttccatcta aaattccgaa
251   aggataaagt gaaggataaa cgtatatata cgtattgaat actatatcaa
301   atgattaatg acgactcgac tgaatctgta ttttttatat gaaaacggaa
351   gaattgttgt gaatagattc cacattgaag aaggaatcga atattcattg
401   atcaaatgat tcactccata gtctgataga tcttttcaag aattgattaa
451   tcggacgaga ataaagatag agtcccgttc tacatgtcaa tgtcggcaac
501   aatgaaattt atagtaagag gaaaatccgt cgactttaaa aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details