JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ458130.1
Database:	nt	Go to original record
Description:	Melanoxerus suavissimus voucher Razafimandimbison 496 (UPS) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Melanoxerus suavissimus
Original size:	1737 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	813	1379	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tnggtatgaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcataa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatctaaaa ttccgaaagg
251   ataaagtgaa ggataaacgt atatatacgt attgaatact atatcaaatg
301   attaatgacg actcgactga atctatattt tttatatgaa aacggaagaa
351   ttggtgtgaa tagattccac attgaagaag gaatcgaata ttcattgatc
401   aaatgattca ctccatagtt tgatagatct tttcaagaat tgattaatcg
451   gacgagaata aagatagagt cccgttctac atgtcaatgt cggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details