JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ458129.1
Database:	nt	Go to original record
Description:	Genipa americana voucher Guedes 30742 (ALCB) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Genipa americana
Original size:	1700 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	732	1315	+	584 bp

Amplicon:

584 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagag
151   aaaccaaagg ttcagaaagt gaaaaaagga taggtgcaga gactcaacgg
201   aagctgttct aacaaaagga gttggctgcg ttagtagaga aatctttcca
251   tctaaaattc cgaaaggata aagtgaagga taaacttata tatacgtatt
301   gaatactata tcagatgatt aatgacgact cgactgaatc tgtatttttc
351   atatgaaaac ggaagaattt gtgtgaatag attccacatt gaagaaagaa
401   tcgaatattc attgatcaaa tgattcactc catagtctga tagatctttt
451   caagaattga ttaatcggac gagaataaag atagagtccc gttctacatg
501   tcaatgtcgg caacaatgaa atttatagta agaggaaaat ccgtcgactt
551   ttaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	584	565	2.63e-08

Match details