JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ458127.1
Database:	nt	Go to original record
Description:	Casasia jacquinioides voucher Rova et al. 2236 (GB) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Casasia jacquinioides
Original size:	1480 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	772	1340	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcattagtag agaaatcttt ccatctaaaa ttccgaaagg
251   ataaagtgaa ggataaacgt atatatacgt attgaatact atatcaaatg
301   attaatgacg actcgactga atctgtattt tttatatgaa aacggaagaa
351   attggtgtga aatagattcc acattgaaga aggaatcgaa tattcattga
401   tcaaatgatt cactccatag tctgatagat cttttcaaga atttattaat
451   cggacgagaa taaagataga gtcccgttct acatgtcaat gtcggcaaca
501   atgaaattta tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details