JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ458126.1
Database:	nt	Go to original record
Description:	Casasia calophylla voucher Rova et al. 2259B (S) tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Casasia calophylla
Original size:	1689 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	744	1311	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccatttt ccgaaaccaa aggttcagaa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt ccatcttaaa attccgaaag
251   gataaagtga aggataaacg tatatatacg tattgaatac tatatcaaat
301   gattaatgat gactcgactg aatctgtatt ttttatatga aaacggaaga
351   attggtgtga atagattcca cattgaagaa ggaatcgaat attcattgat
401   caaatgattc actccatagt ctgatagatc ttttcaagaa ttgattaatc
451   ggacgagaat aaagatagag tcccgttcta catgtcaatg tcggcaacaa
501   tgaaatttat agtaagagga aaatccgtcg acttttaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details