JRC GMO-Amplicons | EURL GMFF

Record ID:	OX335800.1
Database:	nt	Go to original record
Description:	Scutellaria galericulata genome assembly, organelle: plastid:chloroplast
Taxon:	Scutellaria galericulata
Original size:	152565 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48369	48926	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacctactaa gtgataactt tcaaattcag agaaaccccg gaattaataa
101   aaatgggtga tcctgatcca aatcctgttt tctgaaaaca aaggctcaaa
151   aaacgaaaaa tgggataggt gcagagactc gatggaagct gttctaacaa
201   atggagttag ctgcgttgtt agaggaatcc tttccgcgga aacttcagaa
251   aggatgaagg ataaacgtat ctattgaata ctatatcaaa tgattaatga
301   tggcccgaat ctgtattttt gaatatgaaa aacggaagaa tcggtgtgaa
351   ttgattccac attgaaaaaa aaaatggaat attcattcat caaatcattc
401   actccacggt ccaataggtc ttttaaagaa ctaattaatc ggacgagaat
451   aaagatagag tcccattcta catgtcaata ccggcaacaa tgaaatttat
501   agtaagagga aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct
551   ctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details