JRC GMO-Amplicons | EURL GMFF

Record ID:	OL694048.2
Database:	nt	Go to original record
Description:	Plantago asiatica chloroplast, complete genome.
Taxon:	Plantago asiatica
Original size:	164992 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45882	46466	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactagg tgataacttt caaattcaga gaaaccccgg aattaaaaaa
101   aaaaaatggg caatcctgag ccaaatcctg tcttctcaaa ataaaggttc
151   ataaagcgaa aaggggatag gtgcagagac tcgatggaag ctgttctaaa
201   acaaatggag ttgactgcgt tggtagatga atcttttcat cgaaacttca
251   gaaaagatga aggataaacg gatctattga atactaaaat atctaaaaaa
301   aagaaatgac ggcccgagtc tgcatctgta tttttttaga tgaaaaatag
351   aagaattggt gggaattgat tccacattca agaaagaatc gaatattcat
401   tcatcaaatc accccactcc atagtctgat agttttagtc tttttttaaa
451   gaactgatta attaattgga caagaataaa gatagagtcc cgttctacac
501   gtcaataccg acagcaatga aatttatagt aataggaaaa tccgtcgact
551   tttaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details