JRC GMO-Amplicons | EURL GMFF

Record ID:	ON009079.1
Database:	nt	Go to original record
Description:	Anthyllis barba-jovis plastid, complete genome.
Taxon:	Anthyllis barba-jovis
Original size:	151867 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	13254	13841	-	588 bp

Amplicon:

588 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acttaacaag tgaaaacttt caaattcaga gaaaccctgg aattcacaat
101   gggcaatcct gagccaaatc ctgctttacg aaaacaaggg aaagttcagt
151   tcagaaagcg acgagaaaaa aggataggtg cagagactca acggaagctg
201   ttctaacaaa tggagttgac gacacatttc ttttctatgt agaaatctac
251   tttatcaaaa tctatcaaaa ttcgagttcg ggaagggatt gaggagaaaa
301   aatagatata tatgtagaag actctaatat ttattcgatt tgtgaatatt
351   tccatctcaa atgatggatg ttaataaaat caaattcaaa gttaaagaaa
401   aaatagaata ttcattgatc aaatcattca ctccatcgta gttctgatag
451   atcttttgaa aagaactaat caaacgagaa taaagataga gtcccatttt
501   acatgtcaat acgacactaa tgaaattttg agtaagagga aaatccgtcg
551   aattttaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	588	569	2.65e-08

Match details