JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ997431.1
Database:	nt	Go to original record
Description:	Rheum delavayi voucher ZHJ18-31 chloroplast, complete genome.
Taxon:	Rheum delavayi
Original size:	162374 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	50254	50870	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aataaaaaaa
101   tgggcaatcc tgagccaact ccttctttcc aaaaggaaga ataaaaaagg
151   ataggtgcag agactcaatg gaagctgttc taacaaatgg agttgacgat
201   cttgcgttat tataaaaatt cttccatcga aactccaaaa aagatgaaga
251   ataaacctat acgcagacgt acttaaatct taaatataaa tactatatat
301   ataataacta tatattatat aaaaaaatta aattaaaaat taaaaaatat
351   tgatgacgac ccgcattttt ttatgactat gaaaaaatgg aagaattgtt
401   gtgaagcgat tccaagttga agaaagagtc gaatatgcgt ttaataaatt
451   aaagcattta ttccccggtc tgagagatct tttgaagaac caatccatcg
501   gatgagaatg aagatagagt cccattctac gtgtcaatcc tgacaacaat
551   gaaatttata gtaagaggaa aatccgtcga ctttataaat cgtgagggtt
601   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details