JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ997426.1
Database:	nt	Go to original record
Description:	Rheum alexandrae voucher ZHJ18-08 chloroplast, complete genome.
Taxon:	Rheum alexandrae
Original size:	161698 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49857	50452	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aataaaaaaa
101   atgggcaatc ctgagccaac tccttcttta aaaaaggata ggtgcagaga
151   ctcaatggaa gctgttctaa caaatggagt tgacgatctt gcgttattat
201   aaaaattctt ccatcgaaac tccaaaaaag atgaagaata aacctatatg
251   cagacgtact taaatataaa taatatatat aataactata tataatatat
301   tatataaaaa aattaaatta aaaaatattg atgacgaccc gcattttttt
351   tatgactatg aaaaaatgga agaattgttg tgaagcgatt ccaagttgaa
401   gaaagagtcg aatatgcgtt taataaatta aagcatttat tccccggtct
451   gagagatctt ttgaagaacc gatccatcgg atgagaatga agatagagtc
501   ccattctacg tgtcaatcct gacaacaatg aaatttatag taagaggaaa
551   atccgtcgac tttataaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details