JRC GMO-Amplicons | EURL GMFF

Record ID:	OP328201.1
Database:	nt	Go to original record
Description:	Themeda triandra voucher T147_Jobson_2198 chloroplast, complete genome.
Taxon:	Themeda triandra
Original size:	138857 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47314	47838	+	525 bp

Amplicon:

525 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagc ggttctcaaa
151   ctagaaccaa aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcac agaacccata ttataatata
251   ggttctttat tttattttta gaatgaaatt aggaatgatt atgaaataga
301   aaattcataa ttttttgaga attattgtga atctattcca atcgaatatt
351   gagtaatcaa atccttcaat tcattgtttt cgagatcttt taaaaagtgg
401   attaatcgga cgaggataaa gagagagtcc cattctacat gtcaatactg
451   acaacaatga aatttctagt aaaaggaaaa tccgtcgact ttataagtcg
501   tgagggttca agtccctcta tcccc

View other 69 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.36e-08
primerB	100.0	20 bp	0	0	525	506	2.36e-08

Match details