JRC GMO-Amplicons | EURL GMFF

Record ID:	MW802212.1
Database:	nt	Go to original record
Description:	Strobilanthes sp. RMRNR-2022a voucher RMRN_123 chloroplast, complete genome.
Taxon:	unidentified
Original size:	144616 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47643	48192	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaatccaga gaaaccccgg aattaataat
101   aatgggcaat cctgagccaa atcctgtttt ctgaaaaaaa aggtttcgaa
151   aaagaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgact gcaggaatct ttccatcgaa acctcagaaa agatgaagga
251   taaacgtatc tattgaatac tatatcaaat gattagatga cccacatctg
301   tatctgtagt tttttctatg aaaaatggaa gaattgatgt gaattgattc
351   cacattgaag aaagagtcga atattcattc atcaaatcat taactccaaa
401   gtccgatagt tctttaaaag aactgattaa gcggacgaga ataaagatag
451   agtcccattc cacatgtcaa taccggcaac aatgaaattt atagtaagag
501   gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details