JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ573794.1
Database:	nt	Go to original record
Description:	Koenigia mollis var. rudis voucher SD304 chloroplast, complete genome.
Taxon:	Koenigia mollis var. rudis
Original size:	158627 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47520	48110	+	591 bp

Amplicon:

591 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgataatttt caaattcaga gaaaccctgg aattaaaaaa
101   atgggcaatc ctgagccaac tcctgctttc caaaagtaag cataaaaaaa
151   ggataggtgc agagactcaa tggaagctgt tctaacaaat ggagttgact
201   gtcatgcgtt gtgatatgtt ataagaattc ttccataaaa attccaaaaa
251   ggatgaagaa gaaacctcta ggcatacgta cttaactata aatactatat
301   actataatat aaatataata ttgatgacga cccgaatctt tattttatga
351   atatgaacaa ataaaataat tattatgaat cgattccaag ttgaagaaag
401   aatcgaatat tcgtttctta aattaaagca tttactccac agtctgatag
451   atcttttgaa gaaccgatcc gtcggatgag aatgaagata gagtcccatt
501   ctacgtgtca atctcgacaa caatgaaatt tatagtaaga ggaaaatccg
551   tcgactttat aaatcgtgag ggttcaagtc cctctatccc c

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	591	572	2.66e-08

Match details