JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ573786.1
Database:	nt	Go to original record
Description:	Koenigia cyanandra voucher SD428 chloroplast, complete genome.
Taxon:	Koenigia cyanandra
Original size:	155973 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46716	47292	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttaactg ttttgagcct tagtatggaa
51    acctactaag tgataatttt aaaattcaga gaaaccctgg aattaaaaaa
101   aagggcaatc ctgagccaac tcctgctttc caaaagtaag aataaaaaac
151   ggataggtgc agagactcaa tggaagctgt tctaacaaat ggagttgcct
201   ctcaggcatt gttatatgtt atatgaattc ttccatataa acttaaaaag
251   ggatgaagaa gaaacctata ggcatacgta cttaattata aatactatta
301   ttattattat aaatagaatc gacccgaata tttattttat gaataaataa
351   aataattatt acgaatcgat ttcaagttga agaaataata taatattggt
401   ttcttaaaat aaagtattta ttccgcagtc tgatagatct tttgaggaat
451   caacccgttg gatgagaatg aagatagagt cccattctac gtgtcaatct
501   cgacaacaat gaaatttata gtaagaggaa aatccgtcga ttttaaaaat
551   cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details