JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ573784.1
Database:	nt	Go to original record
Description:	Koenigia campanulata var. fulvida voucher SD401 chloroplast, complete genome.
Taxon:	Koenigia campanulata var. fulvida
Original size:	154446 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46038	46649	+	612 bp

Amplicon:

612 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgatcatttt caaattcaga gaaaccctgg aattaaaaaa
101   acgggcaatc ctgagccaac tcctgctttc aaaaagtaag cataaaaaaa
151   ggataggtgc agagactcaa tggaagctgt tctaacaaat ggagttgact
201   gtcatgcgtt gtgatatgtt ataagaattc gtccatataa attcgaacaa
251   ggatgaagaa gaaacctata ggcatacgta cttccctata aatattattt
301   ttcattttta taatttataa atagtaaata gaatattgat atttatgccg
351   acccgaatcg ttattttatg aatatgaaca aataaaataa ttattctgaa
401   tcgattccaa gttgaagaaa gaatcgaata ttcgtttctt aaatgaaagc
451   atttactcca cagtctgata gatcttgtga agaaccgatc catcggatga
501   gaatgaagat agagtcccat tctacgtgtc aatctagaca acaatgaaat
551   ttatagtaag aggaaaatcc gtcgacttta taaatcgtga gggttcaagt
601   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	612	593	2.76e-08

Match details