JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ007410.1
Database:	nt	Go to original record
Description:	Trifolium environmental sample clone IC1P2.7 tRNA-Leu (trnL) gene, partial sequence; plastid.
Taxon:	Trifolium environmental sample
Original size:	610 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	610	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaag
101   gggcaatcct gagccaaatc cttctttccg aaaacaaaat aaaagttcag
151   aaagtgaaaa tcaaaaaggg ataggtgcag agactcaatg gaagctgttc
201   taacaactgg agttgacaac atttcccttg gaagtaggaa aggaatcgtt
251   ctatccaact tccagaaagg aaaagataaa gatatatata acatatatat
301   gtactcaaat actctttcaa ttgattaatg aagacttcta acgtctattt
351   gtaaattgac actatttctt ttctatcaca aatgaaagat ttgaatcaaa
401   tcaattacaa ctggaataaa aaatgaaata ttcattgatc aaatcagtca
451   ctccaccaca gtctgatgaa tcttttgaat aaccgattaa tcagacgaga
501   ataaagatag agtcccattc tacatgtcaa taccgacatc aatgaaattt
551   ttagtaagag gaaaatccgt cgactttaga aatcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details