JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ007409.1
Database:	nt	Go to original record
Description:	Trifolium environmental sample clone IC1P2.2 tRNA-Leu (trnL) gene, partial sequence; plastid.
Taxon:	Trifolium environmental sample
Original size:	621 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	621	+	620 bp

Amplicon:

620 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaag
101   gggcaatcct gagccaaatc cttctttccg aaaacaaaat aaaagttcag
151   aaagttaaaa taaaaaaggg ataggtgcag agactcgatg gaagctgttc
201   taagaagtgg agttgacaac atttccctcg gcagtaggaa aggaatcgtt
251   ctatccaact tccagaaagg aaaagatcaa ggataaacat atatatggta
301   tatatattat gtagtcaaat actctttcaa ttgattaatg aagacttctc
351   acgtctattt gtaaattgta gatatttctt ttctatcaca aatgaaagat
401   ttgaatcaaa tcaattacaa ctggaataaa aaatgaaata ttcattgatc
451   aaatcagtca ctccaccaca gtctgatgaa tcttttgaat agctgattaa
501   tcagacgaga ataaagatag agtcccattc tacatgtcaa taccgacatc
551   aatgaaattt ttagtaagag gaaaatccgt cgactttaga aatcgtgagg
601   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	620	601	2.79e-08

Match details