JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ007383.1
Database:	nt	Go to original record
Description:	Juglans environmental sample clone CotIC7 tRNA-Leu (trnL) gene, partial sequence; plastid.
Taxon:	Juglans environmental sample
Original size:	600 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	600	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattttaaat
101   gggcaatcct gagccaaatc ctgttttcta aaaacaaata agggttcata
151   aagcgagaat caaaaaggat aggtgcagag actcaatgga agctgttcta
201   aaaaatggcg ttgacttcgt tccgttatta aagtaatcct tccatcaaaa
251   ctacagaaag tatgaaggat aaacctatat acatacgtat acgtactgaa
301   atactatctc aaataattaa tgacgacttg aatctttatt tcttttttta
351   tattttttat ttatataaaa actgaaagag ttgttgtgaa tcgatccaaa
401   ttgaagaaag aatcgaatat taattaatca aattatttat tccatcataa
451   tctgatggat cttttgaaga actaattaat cggacgagaa taaagataga
501   gtcccattct acatgtcaat accgacaaca atgaaattta tagtaagagg
551   aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details