JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ123753.1
Database:	nt	Go to original record
Description:	Rhazya stricta chloroplast, complete genome.
Taxon:	Rhazya stricta
Original size:	154736 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48026	48597	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaaacaag ggttcagaaa
151   atgaaaacaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggtc gccttggtct tggtagagaa atctttccat tgaaaattca
251   gaaagaatga agtataaacg tatatacata cgtattgaaa atactatatc
301   aaatgattaa tgcgaacccg aatgaatccg tattttttct ataaaaaatg
351   gaagaattgg tgtgattcga ttccacattg aagaaagaat cgaatattca
401   ttgatcaaat gattcactcc aaagtctgta gatcttttca agaactgatt
451   aatcggacga gaataaagat agagtcccgt tctacatgtc aatgctggca
501   acaatgaaat ttagagtaag aggaaaatcc gtcgacttaa aaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details