JRC GMO-Amplicons | EURL GMFF

Record ID:	OX326955.1
Database:	nt	Go to original record
Description:	Misopates orontium genome assembly, organelle: plastid:chloroplast
Taxon:	Misopates orontium
Original size:	152148 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46507	47083	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtctt ctcaaaacaa aggttcagaa
151   agcgaaaaaa gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgtgttggta gaggaatctt tccatcaaaa cttcagaaaa
251   gatgaaggat aaatctatct aaggataaag ctatctattg aatactatat
301   caaattatta atgatggccc gaatctgtat ctgtattttt ttatatgaaa
351   aatggaagaa ttgatgtgaa ttgattccac attgaagaaa gaatgaaata
401   ttcattcatc aaatcattca ctccatagtc tggtagatct tttaaagaac
451   tgattaatcg gacgagaata aagatagagt cccattctac atgtcaatac
501   cggcaacaat gaaatttata gtaagaggaa aatccgtcga ctttaaaaat
551   cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details